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Image Search Results
Journal: The Journal of Biological Chemistry
Article Title: Curcumin Decreases Amyloid-? Peptide Levels by Attenuating the Maturation of Amyloid-? Precursor Protein
doi: 10.1074/jbc.M110.133520
Figure Lengend Snippet: Curcumin significantly decreases Aβ levels and the ratio of APPma:APPim in mouse primary cortical neurons in a dose-dependent manner. A and B, curcumin significantly decreases both Aβ40 and Aβ42 levels. Mouse primary cortical neurons (E18) were treated with different concentrations of curcumin and harvested after 24 h. Conditioned medium was used in ELISA analysis to detect the Aβ40 and Aβ42 levels, which were normalized to cell numbers. C and D, the curcumin treatment altered APP levels and decreased the ratio of APPma:APPim. In the Western blotting analysis, cell lysates were probed with the APP8717 antibody to reveal APP. β-Actin was used as the loading control. C, a representative gel showing full-length APP and β-actin. D, densitometry of C (n = 3 for each treatment group). Mean ± S.E. *, p < 0.05; **, p < 0.01.
Article Snippet:
Techniques: Enzyme-linked Immunosorbent Assay, Western Blot
Journal: The Journal of Biological Chemistry
Article Title: Curcumin Decreases Amyloid-? Peptide Levels by Attenuating the Maturation of Amyloid-? Precursor Protein
doi: 10.1074/jbc.M110.133520
Figure Lengend Snippet: Curcumin treatment does not decrease cell viability. A and B, H4-APP751 cells (A) or mouse primary cortical neurons (B) were treated with different concentrations of curcumin for 24 h and then subjected to alamarBlue analysis as described under “Experimental Procedures.” Curcumin treatment did not decrease cell viability in either cell type, compared with control (0 μm). The dose of 2.5 μm curcumin significantly increased cell viability by 29.3% in H4-APP751 cells compared with control (A) (p < 0.05) (n = 3 in each treatment group). Mean ± S.E. *, p < 0.05.
Article Snippet:
Techniques: